Upon stimulation by pathogens, netrophils and other leukocytes can undergo NETosis, a programmed cell death leading to the release of decondensed chromatin and antimicrobial molecules. The material released from several neighboring cells forms an extracellular mesh that traps and kills the bacterial, protozoal and fungal pathogens.
More than a decade after its initial description, the accurate vizualization and quantification of NETosis has remained challenging for lack of effective and sensitive tools. Until now.
PlaNET reagents are fluorescent polymer formulations that display exquisite seletively for extruded chromatin. Intact, lysed and necrotic cells are not stained, thus eliminating false positives. Consequently, plaNET reagents allow for clear and accurate visualization of NETosis.
Above: fMLP-treated neutrophils fixed and stained with PlaNET Green and DAPI (40x magnification).
Top: fMLP-treated neutrophils fixed and stained with PlaNET Blue and propidium iodide (10x magnification).
Credits: Patrick McDonald, Université de Sherbrooke.
The dye-conjugated polymer is highly-specific to extruded DNA.
This eliminates cross-reactivity and enables ex-vivo studies with a robust and simple protocol that can be performed in almost any laboratory.